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KMID : 1001020090070020068
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Korean Journal of Urological Oncology
2009 Volume.7 No. 2 p.68 ~ p.73
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Prostate Cancer Killing Effect of Selected Oncolytic Virus Derived from Wyeth Strain Vaccinia Virus
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Kim Tae-Hyo
Yoon Jin-Han
Ha Jung-Min
Ok Yun-chul
Hwang Tae-Ho
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Abstract
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Purpose: With the discovery of recombinant DNA technology, it became possible to genetically engineered viruses to enhance their safety and antitumoral potency. Recently vaccinia virus derived from wyeth strain, JX-594 (TK- and GM-CSF＋), and JX-963, derived from Western Reserve (WR), showed potent antitumor efficacy in some studies. In this study, we have analyzed the effects of JX-596 and JX-963 in TRAMPc-2 cell line.
Materials and Methods: Bioselected method by imposing immunosuppression status was used in VX2 implanted rabbit. JX-594 was used for parent virus. A group of single clone was isolated, amplified and tested. Among these one clone showed strong antitumoral effect after systemic injection, and then this was designated as JX-596. Using JX-596 and JX-963, we evaluated the efficacies of oncolytic virotherapy with prostate cancer by In vitro cytopathic effect and replication assays, biodistribution study.
Results: In vitro cytopathic effect and replication assays, JX viruses made plaques with prostate cancer cell. As the size of viral plaque is associated with the potency of viral spread within in tumor, plaque size induced by JX-596 is more wider and less limit for viral spread suggesting strong bystander effect in vivo. JX-596 IV injection via mouse tail vein showed strong antitumoral efficacy in TRAMPc-2 cell line. Its biodistribution after systemic injection showed tumor specific replication by 10-1000 folds.
Conclusions: In this study, JX viruses showed the efficacies of antitumoral activity with prostate cancer and this result suggests JX-596 has strong antitumoral effects and may be needed for preclinical toxicity study and future clinical application.
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KEYWORD
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Virotherapy, Vaccinia virus, Prostate cancer
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